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以对羟基苯甲醛(HBA)与氯甲基化交联聚苯乙烯(CMCPS)微球表面的氯甲基发生取代反应,将醛基苯基键合在CPS微球表面,形成改性了的BA-CPS微球,然后以其与溶液中的对二甲氨基苯甲醛及吡咯在固-液界面上进行Adler反应,实现了含叔胺基苯基卟啉(TAPP)在交联微球CPS表面的同步合成与固载,再使叔胺基团发生季铵化反应,将TAPP转变为meso-三(p-三甲铵基)苯基卟啉(TMAPP),得到了固载有TMAPP的TMAPP-CPS微球。重点考察了主要因素对TAPP在微球表面的同步合成与固载的影响,并分别以牛血清白蛋白(BSA)与溶菌酶为模型蛋白质,探索了固载化TMAPP与生物大分子形成主-客体作用体系的特性。结果表明,通过固-液相之间的Alder反应及随后的季铵化反应,可以顺利地把TMAPP固载在CPS微球表面且其可与蛋白质分子之间产生强烈的主-客体相互作用。
The substitution reaction of p-hydroxybenzaldehyde (HBA) with chloromethyl on the surface of chloromethylated crosslinked polystyrene (CMCPS) microspheres, the aldehyde phenyl group was bonded to the surface of CPS microspheres to form a modified BA-CPS microspheres were synthesized by Adler reaction with p-dimethylaminobenzaldehyde and pyrrole in the solution at the solid-liquid interface. The results showed that the TAPP containing crosslinked microspheres CPS (TMAPP), and TMAPP (TMAPP) was immobilized on the surface of the membrane by the simultaneous synthesis and immobilization of the surface. Then the quaternization of the tertiary amine group occurred and the TAPP was changed to meso-tris (p- trimethylammonio) phenylporphyrin -CPS microspheres. The effects of main factors on the simultaneous synthesis and immobilization of TAPP on the surface of microspheres were investigated in detail. Bovine serum albumin (BSA) and lysozyme were used as model proteins respectively to explore the effect of immobilized TMAPP and biological macromolecules on the formation of host- The characteristics of the system of object function. The results show that TMAPP can be successfully immobilized on the surface of CPS microspheres through Alder reaction between solid and liquid phase and subsequent quaternization reaction, and it can produce strong host-guest interaction with protein molecules.