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[目的]观察中医辨证方对非酒精性脂肪性肝炎(NASH)大鼠线粒体修复酶的调控作用,探讨其防治NASH的作用机制。[方法]高脂饲料喂养制备NASH大鼠模型,每日灌胃三种中医辨证方(健脾补肾方、活血降浊方、综合方)及易善复进行治疗,10周后检测各组大鼠肝功能、血脂指标,SYBR Green荧光定量PCR法测定肝组织线粒体DNA修复酶,并进行统计。[结果]三种中医辨证方组大鼠肝功能及血脂指标明显低于模型组(P<0.01)且肝脏内线粒体DNA修复酶含量高于正常组(P<0.01)。[结论]三种中医辨证方对NASH大鼠具有良好的治疗效果,可以改善大鼠肝功能及血脂,提高线粒体DNA修复酶含量,促进受损肝细胞自身修复。
[Objective] To observe the regulatory effect of TCM syndrome differentiation on mitochondrial repair enzyme in non-alcoholic steatohepatitis (NASH) rats and to explore its mechanism of action in preventing and treating NASH. [Methods] The NASH rat model was induced by high-fat diet. The rats were treated with three TCM syndrome differentiation prescriptions (Jianpi Bushen Recipe, Huoxue Jiangzhuo Recipe, and Comprehensive recipe) and Yi Shan Fu daily. After 10 weeks, Rat liver function, blood lipid index, SYBR Green quantitative PCR method for determination of mitochondrial DNA repair enzyme in liver tissue, and statistical. [Results] The liver function and blood lipid of the three TCM syndrome differentiation groups were significantly lower than those of model group (P <0.01), and the content of mitochondrial DNA repair enzyme in liver was higher than that of normal group (P <0.01). [Conclusion] The three TCM syndrome differentiation prescriptions have a good therapeutic effect on NASH rats, which can improve liver function and blood lipid in rats, increase the content of mitochondrial DNA repair enzyme and promote the repair of damaged hepatocytes.