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目的 观察用组织培养法保存的兔活性甲状软骨进行同种异体喉软骨移植重建的效果。方法 先取大小约 5mm× 2mm× 1mm兔甲状软骨块 ,分别用RPMI - 1640培养液及 4 %甲醛保存 2 0d ,然后将两种方法保存的软骨块同时移植于同一兔甲状软骨板中线两侧缺损处 ,培养软骨置于左侧 ,甲醛保存软骨置于右侧 ,分别于 7d、14d、30d、60d、90d、12 0d、180d、2 0 0d及 30 0d时取标本观察移植重建效果。结果 用组织培养软骨进行喉移植后与受体组织相容性好 ,移植排斥反应小 ,喉重建修复效果明显优于甲醛保存软骨。结论 用组织培养法保存的活性软骨进行同种异体喉软骨移植其排斥反应小 ,喉重建修复效果满意。
Objective To observe the effect of rabbit allogeneic laryngeal cartilage transplantation with rabbit activated thyroid cartilage preserved by tissue culture. METHODS: Rabbit thyroid cartilage pieces of about 5mm × 2mm × 1mm in size were prepared and stored in RPMI - 1640 culture medium and 4% formaldehyde for 20 days respectively. The cartilage fragments preserved in both methods were simultaneously transplanted on both sides of the same rabbit thyroid cartilage plate Cartilage was placed on the left side of the cartilage placed on the right side of formaldehyde, respectively, at 7d, 14d, 30d, 60d, 90d, 12d, 180d, 200d and 30d specimens were taken to observe the transplant reconstruction effect. Results Tissue culture of cartilage laryngeal transplantation with good compatibility with the recipient tissue, small transplant rejection, throat reconstruction was significantly better than formaldehyde preservation cartilage. Conclusions Allogeneic laryngeal cartilage transplantation with active cartilage preserved by tissue culture method has small rejection and satisfactory thoracoscopic reconstruction.