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目的:探讨银杏叶提取物(EGb)对体外培养的大鼠大脑皮质星形胶质细胞(Ast)增殖周期的影响。方法:体外培养、纯化及鉴定大鼠大脑皮质Ast;MTT比色法选择EGb作用浓度;1×10-7μL·L-1EGb50μL分别加入对照组和实验组培养液;利用流式细胞仪分析技术检测细胞周期。结果:随着Ast培养时间的延长,12h内EGb诱导Ast进入S期+G2/M期的细胞百分率有上升趋势,但24h后稍有下降。结论:EGb可通过影响Ast的细胞周期促进Ast的增殖。
Objective: To investigate the effects of Ginkgo biloba extract (EGb) on the proliferation of cultured rat cerebral cortex astrocytes (Ast). Methods: The rat cerebral cortex Ast was cultured, purified and identified. MTT colorimetric method was used to select the concentration of EGb. 1 × 10-7μL·L-1EGb 50μL was added to the control group and the experimental group respectively. Flow cytometric analysis cell cycle. Results: With the prolongation of Ast culture time, the percentages of cells entering the S phase and the G2 / M phase by EGb in 12 h were increased, but decreased slightly after 24 h. Conclusion: EGb can promote the proliferation of Ast by affecting the cell cycle of Ast.