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目的:建立测定左归丸中马钱苷含量的反相高效液相色谱方法。方法:采用Eclipse XDB-C18色谱柱(4.6mm×250mm,5μm);以四氢呋喃-甲醇-乙腈-水(1∶4∶8∶87)为流动相;流速为1.0mL.min-1;柱温为40℃;检测波长236nm。结果:马钱苷进样浓度在0.0079～0.2530mg.mL-1(r=0.9993)范围内呈良好的线性关系,平均回收率(n=6)为101.6%;RSD为2.1%。结论:该方法简便、重现性好,结果准确可靠,可以用于左归丸中马钱苷的含量测定,为左归丸的质量控制提供一定的理论依据。
Objective: To establish an RP-HPLC method for the determination of loganin in Zuogui pill. Methods: The mobile phase was eluted with Eclipse XDB-C18 column (4.6mm × 250mm, 5μm) with tetrahydrofuran-methanol-acetonitrile-water (1:4:8:87) 40 ℃; detection wavelength of 236nm. Results: The loganin concentration showed a good linearity in the range of 0.0079 ~ 0.2530mg.mL-1 (r = 0.9993) with an average recovery of 101.6% (n = 6) and a RSD of 2.1%. Conclusion: The method is simple, reproducible, and the results are accurate and reliable. It can be used to determine the content of loganin in Zuogui pill and provide a theoretical basis for the quality control of Zuogui pill.