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目的探讨通塞颗粒对慢性阻塞性肺疾病(COPD)急性加重期模型大鼠肺组织细胞外基质的作用。方法将Wistar大鼠随机分为7组,空白组、稳定期模型组、加重期模型组、高剂量组、低剂量组、氨茶碱组、葶贝组,每组10只,以肺炎克雷伯杆菌经鼻腔滴入法建立COPD急性加重期大鼠模型,空白组、COPD稳定期、加重期模型组以生理盐水灌胃,余组以相应剂量药物灌胃,每日2次。放免法监测血清和支气管肺泡灌洗液(BALF)中型前胶原(PC)、层粘连蛋白(LN)、透明质酸(HA)的表达变化,免疫组化法监测肺组织细胞外基质成分的变化。结果血清和BALF中PC、LN、HA表达显示加重期模型组均明显高于稳定期模型组和空白组(P<0.01),稳定期模型组均明显高于空白组(P<0.01);高剂量组均明显低于加重期模型组(P<0.05或P<0.01)。图像分析表明,各组肺组织中型、型、型胶原、LN、TGF-β1的表达均明显高于空白组(P<0.01);加重期模型组、氨茶碱组和高剂量组肺组织肺组织中型、型、型胶原、LN、TGF-β1的表达,低剂量组型、型、型胶原的表达均明显低于加重期模型组(P<0.01)。结论通塞颗粒能抑制COPD急性加重期肺组织细胞外基质成分的降解、气道结构的重塑,以通塞颗粒高剂量组作用最为明显。
Objective To investigate the effect of Tongsai Granules on extracellular matrix in lung tissue of rats with acute exacerbation of chronic obstructive pulmonary disease (COPD). Methods Wistar rats were randomly divided into 7 groups: blank group, stable model group, aggravated model group, high-dose group, low-dose group, aminophylline group, mussel group, 10 in each group. The rat model of acute exacerbation of COPD was established by intranasal instillation of primary bacillus, and the blank group, COPD stable phase, and exacerbation phase of the model group were intragastrically administered with normal saline, and the remaining groups were given intragastric administration of the corresponding dose of the drug twice daily. Radioimmunoassay was used to monitor the expression changes of procollagen (PC), laminin (LN) and hyaluronic acid (HA) in serum and bronchoalveolar lavage fluid (BALF), and the changes of extracellular matrix components in lung tissue were monitored by immunohistochemistry. . Results The expressions of PC, LN and HA in BALF and BALF were significantly higher in the model group than in the stable model group and blank group (P<0.01), and were significantly higher in the stable model group than in the blank group (P<0.01); The dose group was significantly lower than the exacerbation model group (P<0.05 or P<0.01). Image analysis showed that the expression of medium, type, collagen, LN and TGF-β1 in lung tissue of each group was significantly higher than that of the blank group (P<0.01); pulmonary tissue lung in exacerbation phase model group, aminophylline group and high dose group The expression of collagen, LN, and TGF-β1 in medium, type, type, low-dose group, type, and collagen was significantly lower than that in the aggravated model group (P<0.01). Conclusion Tongsai granules can inhibit the degradation of extracellular matrix components and the remodeling of airway structure during the acute exacerbation of COPD. The effect of Tongsai granules in the high dose group is most obvious.