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试验以酶联免疫吸附法为检测基础,在水相中合成发光量子点,再由量子点与磁性纳米材料相结合,形成磁性量子点,然后使磁性量子点与羊抗兔IgG联结制备生物荧光探针。该复合结构物质依靠磁性材料的磁富集作用,促进量子点聚集度,增强量子点荧光信号,利用新方法对目标物进行微量检测,有效地提高了分析测定的灵敏度和稳定性。试验以黄曲霉毒素B_1为模式分析物,检测荧光强度与黄曲霉毒素B_1质量浓度在0.1~1 000 ng/mL范围内成线性关系,检出限为0.1 ng/mL,取得了较满意的结果。
The experiment was based on the enzyme-linked immunosorbent assay (ELISA) as a detection method. The luminescent quantum dots were synthesized in the aqueous phase, then the quantum dot and the magnetic nanomaterials were combined to form magnetic quantum dots. Then, the magnetic quantum dots Probe. The composite structure relies on the magnetic enrichment of magnetic materials to promote the aggregation degree of quantum dots and enhance the fluorescent signal of the quantum dots. The novel method is used for the trace detection of the target, which effectively improves the sensitivity and stability of the analysis. In the experiment, aflatoxin B 1 was used as the model analyte. The linear relationship between the detection fluorescence intensity and aflatoxin B 1 mass concentration ranged from 0.1 to 1 000 ng / mL with a detection limit of 0.1 ng / mL. Satisfactory results were obtained .