目的 :建立血浆中硝苯地平的高效液相色谱测定方法。方法 :取血浆 1.0ml,以尼群地平为内标 ,用乙酸乙酯及环己烷 (3 1)提取两次 ,有机相在5 5℃水浴中氮气吹干 ,残渣用流动相复溶后进样分析。结果 :本法在 5～ 2 0 0 μg·L-1范围内线性良好 ,相关系数r =0 .9999(n =6)。高、中、低三种浓度硝苯地平的回收率为 90 .13%～ 99.4 3% ,日内精密度为 1.4 3%～ 6.61% ,日间精密度为 3.85 %～8.79% ,硝苯地平和内标的平均绝对回收率分别为90 .5 8%和 91.5 9%。内源性物质对测定无干扰。结论 :本法灵敏度及特异性高 ,操作简便 ,适用于硝苯地平的临床药代动力学研究 Objective: To establish a method for the determination of nifedipine in plasma by high performance liquid chromatography. Methods: Take 1.0 ml of plasma and use nitrendipine as internal standard. Extract with ethyl acetate and cyclohexane (3 1) twice. Dry the organic phase in a water bath at 55 ° C and dry with nitrogen. After the residue is reconstituted with the mobile phase Injection analysis. Results: The method was linear in the range of 5 ~ 200 μg · L-1 with a correlation coefficient of r = 0.9999 (n = 6). The recoveries of high, middle and low concentrations of nifedipine were 90.13% -99.4 3%, intra-day precision was 1.4 3% -6.61%, intra-day precision was 3.85% -8.79%, nifedipine and The average absolute internal standard recoveries were 90.58% and 91.59% respectively. Endogenous substances have no interference with the assay. Conclusion: This method is sensitive and specific, easy to operate, suitable for clinical pharmacokinetics of nifedipine