以每次剂量为体重的5×10－6（m／m）17α－甲基睾酮（17α－MT）投喂2～4龄赤点石斑鱼，成功地促使其提早3～4a性转变为具有生殖功能的雄性鱼。经50d投喂药饵46次，累积总剂量达到鱼体重的241．3×10－6（m／m）时，性腺中的卵巢组织萎缩，绝大部分卵母细胞已退化；精巢组织发达，间质细胞数量增多，精小囊内充满成熟精子，雄性化率达100％。实验组的流精率为93．5％。雄性化后的平均成熟系数为2．30％，与自然海区的相近似。精子寿命90min，活率94．6％。激素诱导的精液与催产诱导雌性鱼成熟卵子进行人工授精，受精率81．1％，胚胎发育正常，受精后23h30min孵出仔鱼，孵化率76．7％。证实了经外源激素诱导雄性化的赤点石斑鱼精子具有受精能力。 The 2nd to 4th instar red grouper fed 5 × 10-6 (m / m) 17α-methyltestosterone (17α-MT) at each dose successfully successfully transformed them into 3- Male fish with reproductive function. Ovarian tissue in the gonads atrophy and most of the oocytes have been degenerated when the cumulative total dose reaches 241.3 × 10-6 (m / m) after 50 d feeding. Increased number of interstitial cells, sperm sac filled with mature sperm, male rate of 100%. The flow rate of the experimental group was 93.5%. The average maturity of males is 2.30%, similar to the natural sea area. Sperm life 90min, 94.6% viability. Hormone-induced semen and induced ovulation induced female fish mature eggs for artificial insemination, fertilization rate of 81.1%, normal embryonic development, hatching larvae after 23h30min fertilization, hatching rate of 76.7%. Confirmed that exogenous hormones induced males of red spot grouper sperm fertilization.