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目的研究外源绿色荧光蛋白(green fluorescent protein,简称GFP)基因在BALB/c绿色荧光裸鼠主要器官组织中的表达及其差异。方法小动物成像系统和RT-PCR方法检测GFP的组织分布以及荧光表达水平情况。结果经活体荧光影像系统观察及PCR方法检测发现GFP可以在裸鼠多个器官组织中表达,其中在胰腺、心脏、全脑、皮肤、睾丸中表达量较高。结论外源绿色荧光蛋白可以在模型动物体内成功表达且稳定遗传,其中在胰腺组织中高表达。
Objective To study the expression and difference of green fluorescent protein (GFP) gene in the main organs and tissues of BALB / c green fluorescent nude mice. Methods Small animal imaging system and RT-PCR method were used to detect the tissue distribution and fluorescence expression of GFP. Results In vivo fluorescence imaging system and PCR assay showed that GFP was expressed in multiple organs and tissues of nude mice, especially in the pancreas, heart, whole brain, skin and testes. Conclusion Exogenous green fluorescent protein can be expressed successfully and stably in the model animal, which is highly expressed in pancreatic tissues.