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目的 新生儿窒息可引起糜烂、溃疡和出血等胃肠粘膜损伤。既往成年动物研究发现表皮生长因子 (EGF)及其受体 (EGFR)在急性胃肠粘膜损伤后的修复过程中发挥重要作用。该文旨在探讨EGF和EGFR在宫内窒息新生鼠胃粘膜的表达及其在胃粘膜损伤后修复过程中的作用。方法 用孕足 2 1d大鼠制作成宫内窒息模型 ,将剖宫产后存活的窒息组和对照组新生鼠在出生 0、2 4、4 8和 72h断头处死 ,留取胃标本。应用免疫组化方法和反转录 -多聚酶链反应动态监测新生鼠胃粘膜EGF、EGFR和EGFRmRNA的表达 ,同时观察胃组织形态学改变。结果 (1 )窒息组新生鼠出现胃粘膜损伤 ,于生后 4 8h损伤最重 ,之后开始恢复。 (2 )对照组新生鼠胃粘膜有较弱的EGF和EGFR表达 ,各时间点差异无显著性 (P >0 .0 5 )。 (3)窒息组新生鼠胃粘膜EGF和EGFR的表达随生存时间增加而增强 ,在 4 8h达高峰之后开始下降 ,生后 2 4 ,4 8,72h胃粘膜EGF表达与对照组同时相比较 ,差异均有显著性 (P <0 .0 1或 0 .0 5 )。 (4 )窒息组新生鼠胃粘膜EGFRmRNA的表达于 2 4h达高峰 ,较对照组同时间点相比增加 ,差异非常显著性 (P <0 .0 1 ) ,之后开始减少。结论 EGF和EGFR可能在窒息后新生鼠胃粘膜损伤后的修复过程中发挥重要作用
The purpose of neonatal asphyxia can cause erosion, ulcers and bleeding gastrointestinal mucosal injury. Previous studies in adult animals found that epidermal growth factor (EGF) and its receptor (EGFR) play an important role in the repair of acute gastrointestinal mucosal injury. This article aims to investigate the expression of EGF and EGFR in neonatal rat gastric mucosa after intrauterine asphyxia and its role in the repair process after gastric mucosal injury. Methods The pregnant rats of 21 days old were made into an asphyxia model. The asphyxia survived cesarean section and the control group were sacrificed at 0, 2, 4, 8 and 72 hours after birth, and their gastric samples were collected. The expression of EGF, EGFR and EGFR mRNA in gastric mucosa of neonatal rats was dynamically monitored by immunohistochemistry and reverse transcription-polymerase chain reaction (RT-PCR). Morphological changes of gastric tissues were also observed. Results (1) Gastric mucosal injury occurred in neonatal rats in asphyxia group, with the heaviest injury 48 hrs after birth, and then began to recover. (2) The expression of EGF and EGFR in the gastric mucosa of neonatal rats in the control group was weaker than that in the control group, with no significant difference at each time point (P> 0.05). (3) The expression of EGF and EGFR in gastric mucosa of neonatal rats in asphyxia group increased with the increase of survival time, and then decreased after reaching the peak at 48h. Compared with the control group, EGF expression in gastric mucosa at 24, 48, The difference was significant (P <0.01 or 0.05). (4) The expression of EGFRmRNA in gastric mucosa of neonatal rats in asphyxia group reached a peak at 24 hours, which was significantly higher than that in the control group at the same time point (P <0.01), then began to decrease. Conclusion EGF and EGFR may play an important role in the repair of gastric mucosal injury after asphyxia in neonatal rats