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本文研究高压和高压氧对小鼠大脑碱性磷酸酶等6种酶的活性影响。30只小鼠随机分为空白对照组、高压组和高压氧组。高压组动物置于加压舱内,高压氧组动物置于同一加压舱中的小套舱内,分别给予5ATA的空气和纯氧,停留37分钟。高压处理结束,按组织化学要求取材、固定、切片和酶活性反应。实验结果表明:高压氧可致动物小痉挛;高压环境对动物机体无明显刺激影响;高压氧使碱性磷酸酶(AKP)、葡萄糖-6-磷酸酶(G-6-P)、乳酸脱氢酶(LDH)、乙酰胆碱酯酶(AChE)活性增强,高压环境使上述4种酶活性略有增强,和空白对照组比差别不明显。高压和高压氧使单胺氧化酶(MAO)和过氧化物酶活性略有降低。本文对高压氧环境导致机体行为变化和6种酶活性改变的意义作了讨论。
This article studies the effects of hyperbaric and hyperbaric oxygen on the activity of 6 enzymes such as alkaline phosphatase in mouse brain. Thirty mice were randomly divided into blank control group, high pressure group and hyperbaric oxygen group. The animals in the high-pressure group were placed in a pressurized chamber, and the hyperbaric oxygenated animals were placed in a small cabin in the same pressurized chamber, respectively giving 5ATA of air and pure oxygen for a stay of 37 minutes. High-pressure treatment is completed, according to the requirements of tissue chemistry drawn, fixed, sliced and enzyme activity. The experimental results showed that: hyperbaric oxygen can cause convulsion in animals; high-pressure environment has no obvious stimulus on animal body; hyperbaric oxygen causes alkaline phosphatase (AKP), glucose-6-phosphatase (G-6-P) Enzyme (LDH), acetylcholinesterase (AChE) activity increased, high-pressure environment so that the above-mentioned four kinds of enzyme activity increased slightly, compared with the blank control group, the difference was not obvious. High pressure and hyperbaric oxygen slightly decreased monoamine oxidase (MAO) and peroxidase activity. This article discusses the role of hyperbaric oxygen environment lead to changes in body behavior and changes in the significance of six enzymes are discussed.