[背景]人类在生命早期的发育期间可能特别容易受到环境暴露的影响。有关这一主题的人体研究一般集中在单一暴露与健康效应之间的关系。而“暴露组”的概念涵盖了从受孕开始的的全部暴露,完善了基因组。[目的]人类生命早期暴露组(HELIX)项目是一个新的合作研究项目,目的是为了采用新的暴露评估和生物标志分析方法来描述生命早期多种环境因素的暴露,并将这些与生物标志组学和儿童健康结局相关联,从而刻画“生命早期暴露组”。本文描述了该项目的总体设计。[方法]HELIX将利用欧洲现有的6个出生队列研究来估计产前、产后暴露的一系列化学和物理暴露。建立全部队列中总共32 000对母亲和儿童的暴露模型,并在一个包含1 200对母亲和儿童的子队列中测量生物标志。嵌套重复采样的定组研究(n=150)将收集生物标志的变化数据,利用智能手机来评估流动性和体力活动,并监测个体暴露。采用组学技术确定与暴露相关的分子学特征(代谢组、蛋白质组、转录组和表观基因组)。对于多次暴露,采用统计方法估计胎儿和儿童的成长、肥胖、神经发育和呼吸系统结局中的暴露-效应关系。项目还将进行一项健康效应评估测试,以评价组合暴露的风险和收益。[结论]HELIX是描述欧洲人群生命早期暴露组并解开它与组学标志物和儿童健康之间关联的首次尝试之一。作为对暴露组学这一概念的验证,该项目向生命过程中暴露组迈出了重要的第一步。 [Background] Humans may be particularly vulnerable to environmental exposure during the early stages of their life. Human studies on this topic have generally focused on the relationship between single exposure and health effects. The concept of “exposure group ” covers the entire exposure from the beginning of conception and improves the genome. [Purpose] The HELIX project is a new collaborative research project to introduce new exposure assessment and biomarker analysis methods to describe exposure to multiple environmental factors early in life and to link these with biomarkers Group learning and child health outcomes associated, thus characterizing “early life group ”. This article describes the overall design of the project. [Method] HELIX will use the existing 6 birth cohort studies in Europe to estimate a series of chemical and physical exposures to prenatal and postpartum exposures. A total of 32,000 models of exposure to mothers and children in the entire cohort were established and biomarkers were measured in a sub-cohort of 1,200 mothers and children. Nested resampling-based studies (n = 150) will collect biomarker change data, use smartphones to assess mobility and physical activity, and monitor individual exposures. Using omics techniques to determine the molecular characteristics associated with exposure (metabolome, proteome, transcriptome, and epigenome). For multiple exposures, statistical methods were used to estimate exposure-effect relationships in fetal and child growth, obesity, neurodevelopment, and respiratory outcomes. The project will also conduct a health effects assessment test to assess the risks and benefits of the combined exposure. [Conclusion] HELIX is one of the first attempts to describe the association between early exposure to life in European populations and its association with histological markers and child health. As a validation of the concept of exposure biology, this project has taken an important first step towards exposing life processes.