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目的:研究黄芪水煎煮过程中产生的固体微粒对活性成分黄芪甲苷在体肠吸收的影响。方法:黄芪经常规水煎煮后除净药渣,以3 000、6 000、12 000 r/min梯度离心法分离水煎液中的固体微粒,以在体肠循环灌注试验评价固体微粒对黄芪甲苷肠吸收的影响。结果:经梯度离心所获固体微粒粒径分别为455.2±22.51 nm、382.7±21.76 nm、218.8±20.43 nm;将不同粒径的固体微粒加入含黄芪甲苷的K-R液中作为灌流液,可显著提高黄芪甲苷的单位肠面积吸收量、单位肠面积吸收率与肠壁通透性,并且随着粒径的降低,黄芪甲苷的单位肠面积吸收量、吸收率与肠壁通透性都显著性提高(P<0.05)。结论:黄芪水煎煮过程中形成的固体微粒可显著影响活性成分黄芪甲苷的肠吸收性能,且微粒粒径越小促活性成分吸收作用越强。
Objective: To study the effect of solid particles produced by Astragalus membranaceus decoction on intestinal absorption of astragaloside IV in the body. Methods: The Astragalus membranaceus was separated from the drug residue by conventional water decoction. The solid particles in the decoction were separated by gradient centrifugation at 3 000, 6 000 and 12 000 r / min. Effect of Adenosine on intestinal absorption. Results: The diameters of the solid particles obtained by gradient centrifugation were 455.2 ± 22.51 nm, 382.7 ± 21.76 nm and 218.8 ± 20.43 nm, respectively. When solid particles with different particle sizes were added into the KR fluid containing astragaloside IV as the perfusate, Astragaloside increased per unit area of ?? intestinal absorption, unit area of intestinal absorption rate and intestinal wall permeability, and with the decrease of particle size, the unit area of ?? intestinal absorption of Astragaloside, the absorption rate and intestinal permeability are Significantly increased (P <0.05). Conclusion: The solid particles formed during the boiling process of Astragalus membranaceus significantly affect the intestinal absorption of the active ingredient Astragaloside IV, and the smaller the particle size, the stronger the absorption of the active ingredient.