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在真核生物中,DNA缠绕在组蛋白上形成核小体,一个组蛋白分子包括H2A、H2B、H3和H4各2个核心组蛋白亚基。在这4种核心组蛋白中,H2A富含多样化,且在细胞的生物途径中起重要作用的变异体,因此,H2A家族一直是研究热点。致病疫霉是重要的病原菌也是研究卵菌的模式物种之一,目前关于卵菌表观遗传的研究还未见报道。本研究针对致病疫霉组蛋白H2A变异体,利用基因组信息和基因芯片数据,通过序列比对、系统发育分析以及基因表达水平检测,发现在致病疫霉基因组中存在组蛋白H2A变异体H2A.X.1、H2A.X.2和H2A.Z,它们在不同生长发育阶段和侵染过程呈现特异的表达谱。研究结果为进一步研究致病疫霉表观遗传机制奠定了基础。
In eukaryotes, DNA is entangled in histones to form nucleosomes, and one histone molecule includes two core histone subunits, H2A, H2B, H3 and H4. Among the four core histones, H2A is rich in diversity and plays an important role in cellular biological pathways. Therefore, the H2A family has been a hotspot of research. Phytophthora infestans is one of the important pathogens and one of the model species for studying Oomycetes. At present, there are no reports on the epigenetic studies on oomycetes. In this study, H2A variant of histone H2A was found in Phytophthora infestans genome by sequence alignment, phylogenetic analysis and gene expression analysis using genomic information and gene chip data. .X.1, H2A.X.2 and H2A.Z, which showed specific expression profiles at different stages of development and infection. The results laid the foundation for further study on the epigenetic mechanism of Phytophthora infestans.