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目的研究宫颈人乳头瘤病毒(HPV)感染与宫颈组织硒含量的关系。方法用第2代捕获杂交(HC2)法确定宫颈的HPV感染,再用基因分型检测系统确定其HPV亚型;荧光光度技术检测宫颈组织硒的含量。结果HPV感染组宫颈组织硒含量:118例慢性宫颈炎组为(5.33±2.32)mmol/g,宫颈上皮内瘤变(CIN)Ⅰ级组(83例)为(5.29±2.21)mmol/g,CINⅡ级组(44例)为(3.19±1.87)mmol/g,CINⅢ级组(31例)为(2.73±1.64)mmol/g。组间比较差异有显著性(P<0.05);278例HPV阴性对照宫颈组织硒的平均含量为(6.03±3.29)mmol/g,与HPV感染组比较差异有显著性(P<0.01)。结论硒含量与宫颈组织的HPV感染有关,其下降程度与病变程度相一致,补充适量的微量元素硒可能有利于CIN恢复。
Objective To study the relationship between human papillomavirus (HPV) infection and selenium content in cervical tissue. Methods Cervical HPV infection was determined by the second generation hybridization (HC2) method. The HPV subtypes were identified by genotyping detection system. Fluorescence spectrophotometry was used to detect the content of selenium in cervical tissue. Results Selenium content in cervical tissue of HPV infection group was (5.33 ± 2.32) mmol / g in 118 cases of chronic cervicitis group and (5.29 ± 2.21) mmol / g in 83 cases of cervical intraepithelial neoplasia group (83 cases) The CINⅡgroup (44 cases) was (3.19 ± 1.87) mmol / g, while the CINⅢgroup (31 cases) was (2.73 ± 1.64) mmol / g. There was significant difference between the two groups (P <0.05). The average content of selenium in 278 cases of HPV negative control group was (6.03 ± 3.29) mmol / g, which was significantly different from that of HPV infection group (P <0.01). Conclusion The selenium content is related to the HPV infection in cervical tissue, and the degree of selenium is consistent with the lesion degree. Supplementation of trace element selenium may be beneficial to the recovery of CIN.