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目的明确水通道蛋白(AQP)1和微血管密度(MVD)在子宫颈上皮内瘤样变(CIN)和子宫颈鳞癌、腺癌组织中的表达和分布,探讨其在子宫颈癌发病中的意义。方法采用免疫组织化学Envision法检测AQP1和MVD在74例子宫颈癌(其中子宫颈鳞癌46例,子宫颈腺癌28例),34例CIN和15例正常子宫颈组织中的表达情况。结果AQP1主要表达于CIN和子宫颈鳞癌、腺癌的血管内皮细胞,以腺癌表达量最大,鳞癌与CIN的表达量基本一致,而在其他各组间都有统计学意义。随着宫颈病变的进展,MVD逐渐增大,各组间比较差异有统计学意义。但发现在子宫颈鳞癌、腺癌和正常组织中MVD的表达量要比AQP1大,并有统计学意义;而在CIN中的表达量相对一致。在子宫颈癌的临床病理特征中,淋巴转移阳性者AQP1和MVD的表达量较阴性者高(P<0.01)。结论AQP1主要表达于子宫颈癌组织间的血管内皮细胞,其表达量较微血管密度低,并与微血管一起在子宫颈癌的生长、侵袭和转移中起重要的作用。
Objective To investigate the expression and distribution of aquaporin-1 (AQP-1) and microvessel density (MVD) in cervical intraepithelial neoplasia (CIN) and cervical squamous cell carcinoma and adenocarcinoma and to explore its significance in the pathogenesis of cervical cancer . Methods The expression of AQP1 and MVD in 74 cases of cervical cancer (including 46 cases of cervical squamous cell carcinoma and 28 cases of cervical adenocarcinoma), 34 cases of CIN and 15 cases of normal cervical tissue were detected by immunohistochemical Envision method. Results AQP1 was mainly expressed in vascular endothelial cells of CIN, cervical squamous cell carcinoma and adenocarcinoma. The expression of AQP1 was the highest in adenocarcinoma, and the expression of squamous cell carcinoma was the same as that in CIN. However, it was statistically significant in other groups. With the progress of cervical lesions, MVD gradually increased, the difference between the groups was statistically significant. However, the expression of MVD in squamous cell carcinoma of the cervix, adenocarcinoma and normal tissues was found to be larger than AQP1 and statistically significant, while it was relatively consistent in CIN. In the clinicopathological features of cervical cancer, the expression of AQP1 and MVD in lymph node positive patients was higher than that in negative patients (P <0.01). Conclusions AQP1 is mainly expressed in vascular endothelial cells between cervical cancer tissues. The expression of AQP1 is lower than that of microvessel and plays an important role in the growth, invasion and metastasis of cervical cancer together with microvessels.