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以黑暗培养为对照,以白光、红光、蓝光、黄光、UV-A和UV-B为处理,研究了不同光质对大豆芽苗菜生长和抗坏血酸含量的影响。结果表明:与对照相比,光照处理显著降低了大豆芽苗菜下胚轴的长度(白光除外),且显著提高了下胚轴和子叶中抗坏血酸含量。与其它光质处理相比,UV-A连续光照36 h后,下胚轴和子叶中抗坏血酸含量提高最显著。进一步着重研究了UV-A的调节机理,与对照相比,UV-A连续光照36 h后,大豆芽苗菜子叶中谷胱甘肽含量显著升高,下胚轴和子叶中DHAR、GR酶活性及其基因的相对表达量均显著提高。综上所述,光照有利于大豆芽苗菜中抗坏血酸含量的积累;UV-A可能是通过提高DHAR、GR酶活性及基因的表达量显著提高抗坏血酸的含量。
The effects of different light qualities on the growth and the content of ascorbic acid in soybean sprouts were studied with darkness as control, white light, red light, blue light, yellow light, UV-A and UV-B treatments. The results showed that the light treatment significantly reduced the hypocotyl length of soybean sprouts (except white light) and significantly increased the content of ascorbic acid in hypocotyls and cotyledons compared with the control. Compared with other treatments, the content of ascorbic acid in hypocotyls and cotyledons increased most significantly after UV-A irradiation for 36 h. Further studies focused on the regulation mechanism of UV-A. Compared with the control, the glutathione content in the leaves of soybean sprouts increased significantly after 36 hours continuous UV-A irradiation, while the activities of DHAR and GR in the hypocotyls and cotyledons And their relative gene expression were significantly increased. In conclusion, UV-A may promote the accumulation of ascorbic acid in soybean sprouts. UV-A may significantly increase the content of ascorbic acid by increasing the activity of DHAR and GR and the gene expression.