目的观察高糖环境对内皮祖细胞(EPC)的损伤作用及罗格列酮对其的保护作用。方法将健康志愿者骨髓单个核细胞接种于包被有人纤维黏连蛋白的培养板中,培养7天后获得正分化的EPC。贴壁细胞随机分为对照组、高糖组和高糖加罗格列酮组,分别于24h、96h后MTT测定3组细胞增殖功能,PI单染法检测其凋亡率。结果高糖作用24h促进EPC的增殖能力,与对照组比,其OD值更大(P<0.05),且凋亡率没有明显增加(P>0.05)。96h后,高糖明显抑制其增殖能力,OD值低于对照组(P<0.05),且凋亡率明显增加(P<0.01)。罗格列酮干预后OD值比高糖组明显增加(P<0.05),且凋亡率明显降低(P<0.01)。结论长期高糖作用可抑制EPC增殖功能,使凋亡率增加;罗格列酮可能保护高糖环境下EPC的增殖能力并降低凋亡率。 Objective To observe the injury of endothelial progenitor cells (EPCs) induced by high glucose and the protective effect of rosiglitazone on EPCs. Methods Bone marrow mononuclear cells from healthy volunteers were inoculated into human fibronectin-coated plates and cultured for 7 days to obtain differentiated EPCs. Adherent cells were randomly divided into control group, high glucose group and high glucose plus rosiglitazone group. MTT assay was used to detect the proliferation of the three groups at 24h and 96h, respectively. The apoptosis rate was detected by PI single staining. Results The effect of high glucose on the proliferation of EPC was higher than that of the control group (P <0.05), and the apoptosis rate did not increase significantly (P> 0.05). After 96h, the high glucose significantly inhibited the proliferation, the OD value was lower than the control group (P <0.05), and the apoptosis rate was significantly increased (P <0.01). Compared with high glucose group, the OD value of rosiglitazone significantly increased (P <0.05) and the apoptosis rate decreased significantly (P <0.01). Conclusion Long-term high glucose can inhibit the proliferation of EPC and increase the apoptosis rate. Rosiglitazone may protect the EPC proliferation and decrease the apoptosis rate under high glucose conditions.