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对脉动真空灭菌过程选用１３２℃２分钟、１３２℃４分钟、１２８℃３０分钟的３种条件，用１３２℃压力蒸气灭菌指示管、３Ｍ指示胶带、留点温度计、嗜热脂肪杆菌芽胞进行对照实验观察，以嗜热脂肪杆菌芽胞灭菌后培养无菌生长为合格标志。结果表明１３２℃２分钟、４分钟条件下指示管指出结果与嗜热脂肪杆菌芽胞菌片结果相符，但２分钟条件下指示管与嗜热脂肪杆菌芽胞菌片均指示灭菌不合格，而４分钟灭菌条件指出的结果为合格，指示管能指示灭菌状态时的温度和所持续的时间，留点温度计只能指示灭菌状态时达到的温度，指示管不适用于低于１３２℃以下温度条件的压力蒸气灭菌器。
Two conditions of 132°C for 2 minutes, 132°C for 4 minutes, and 128°C for 30 minutes were selected for the pulsatile vacuum sterilization process. They were performed with a 132°C pressure steam sterilization indicator tube, 3M indicator tape, retention thermometer, and Bacillus stearothermophilus spores. Controlled experimental observations showed that sterile growth of cultures after sterilization with Bacillus stearothermophilus spores was a qualified marker. The results showed that the indicator tube indicated that the results were consistent with the results for the Bacillus stearothermophilus bacillus tablet at 132°C for 2 minutes and 4 minutes, but the indicator tube and the Bacillus stearothermophilus bacillus sheet showed sterilization failure at 2 minutes, and 4 The minute sterilization condition indicates that the result is acceptable, indicating that the tube can indicate the temperature and duration of the sterilization state, leaving the thermometer to indicate only the temperature reached in the sterilization state, indicating that the tube is not suitable for temperatures below 132°C. Pressure condition steam sterilizer at temperature conditions.