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本研究基于巨桉全基因组的108个SSR标记(44个基因SSRs和64个EST-SSRs),通过对细叶桉9个群体的78株样品的初步关联分析和242株样品的关联验证,利用一般线性模型和混合线性模型共检测到与细叶桉9月生树高(H9)、30月生树高(H30)和30月生胸径(H_(30))显著关联的标记分别为2个、3个和4个。除1个标记(Embra227)同时与H30和H_(30)显著关联外,不同性状的关联标记均不相同。单个标记对表型变异的解释率为10.3%~34.6%。与H9、H_(30)和D_(30)关联的最大增效等位片段的效应值分别为0.67 m、1.64 m和1.97 cm,最大减效等位片段的效应值分别为-0.72 m、-1.94 m和-1.87 cm。H9、H30和H_(30)的平均增效效应最大的关联标记分别为EUCe SSR1070(0.41 m,26.8%)、EUCe SSR1136(0.73 cm,12.3%)和EUCe SSR906(1.48 cm,25.3%),平均减效效应最大的关联标记分别为EUCe SSR1070(-0.25 m,16.3%)、Embra227(-1.06 m,17.2%)和Embra227(-1.15 cm,17.7%)。这为桉树分子育种提供了有潜力的标记资源。
Based on the 108 SSR markers (44 SSRs and 64 EST-SSRs) of Eucalyptus grandis genome-wide genome, this study was based on the preliminary association analysis of 78 samples from 9 populations of E. grandis and the correlation analysis of 242 samples. Markedly associated with H9, H30 and H_ (30) of 9-month-old Eucalyptus urophylla were detected by general linear model and mixed linear model, respectively , 3 and 4. Except for one marker (Embra227), which was significantly associated with H30 and H_ (30), the markers of different traits were different. A single marker on the phenotypic variation explained 10.3% ~ 34.6%. The maximum synergistic alleles associated with H9, H_ (30) and D_ (30) had effects of 0.67 m, 1.64 m and 1.97 cm, respectively, and the effects of the maximal alleles were -0.72 m, 1.94 m and -1.87 cm. The correlation markers with the highest average synergistic effects on H9, H30 and H_ (30) were EUCe SSR1070 (0.41 m, 26.8%), EUCe SSR1136 (0.73 cm, 12.3%) and EUCe SSR906 (1.48 cm, 25.3% The most significant correlative markers of reduction effect were EUCe SSR1070 (-0.25 m, 16.3%), Embra227 (-1.06 m, 17.2%) and Embra227 (-1.15 cm, 17.7%) respectively. This provides a potential marker resource for molecular breeding of eucalyptus.