提出用荧光光谱法测定环糊精包络物离解常数的新方法。对于1:1型包络物,若客体与包络物均有荧光且波长相同或者客体无荧光或很弱,包络物有荧光,则可用公式(I-I_0)/(I_∞-I)=(I/K_d)[CD]测定离解常数K_d 用提出的方法测定了对羟基苯甲酸和蒽与β-环糊精在水溶液中包络物的离解常数,与文献报道值一致。 A new method to determine the dissociation constant of cyclodextrin inclusion by fluorescence spectroscopy was proposed. For the 1: 1 envelope, if both the guest and the envelope have fluorescence and the same wavelength or the guest is non-fluorescent or weak, and the envelope has fluorescence, the formula (I-I_0) / (I_∞-I) = (I / K_d) [CD] Determination of dissociation constant K_d The proposed method was used to determine the dissociation constant of p-hydroxybenzoic acid and anthracene with β-cyclodextrin in aqueous solution.