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应用高效液相色谱－紫外检测技术检测了大鼠全脑缺血１５ｍｉｎ及再灌流１ｈ～７ｄ海马、皮层下（丘脑和纹状体）、新皮层突触体（Ｚａｌｅｓｋａ方法）主要磷脂组分含量变化。结果显示，短暂缺血再灌流后各脑区存在着磷脂代谢的异常，再灌流期磷脂的降解甚于缺血期。磷脂组分中以ＰＥ、ＰＣ的减少为著，在ＰＥ、ＰＣ显著减少时也存在ＰＳ的减少；而ＳＭ无明显减少，随再灌流的延长有升高趋势。３个脑区磷脂含量的减少存在差别，反映了激动剂依赖性磷脂降解以及Ｃａ￣２＋依赖性磷脂酶活化的区域性，是选择性神经元损伤的生化基础。ＰＣ／ＳＭ比值的缩小可能更敏感地提示了膜损害或修复过程中膜结构的变化。
The contents of major phospholipid components in hippocampus, subcortical (thalamus and striatum) and neocortex synaptosomes (Zaleska method) were detected by high performance liquid chromatography-ultraviolet detection after 15 min of global cerebral ischemia and 1 h to 7 d of reperfusion Variety. The results showed that there was an abnormal phospholipid metabolism in various brain regions after transient ischemia and reperfusion, and the degradation of phospholipid during reperfusion was more than that of ischemia. In the phospholipid fraction, the decrease of PE and PC was observed, and there was also a decrease of PS when PE and PC were significantly reduced. However, SM did not decrease obviously, but increased with the prolongation of reperfusion. There is a difference in the reduction of phospholipid content in the three brain regions, reflecting the degradation of agonist-dependent phospholipids and the regionalization of Ca 2+ -dependent phospholipase activation, which is the biochemical basis of selective neuronal damage. The shrinkage of the PC / SM ratio may suggest more sensitively the changes in membrane structure during membrane damage or repair.