70年代以来,茶树组织培养作为快速繁殖和遗传育种的一项重要手段在茶树育种研究领域得到广泛的重视,许多中外科学家用茶籽子叶、成熟胚、未成熟胚、花药、叶柄、茎皮层等器官和组织作外植体,通过培养,均获得了完整植株.在实际培养过程中,往往先诱导芽苗分化.然后将分化的芽苗转入含有低浓度生长素或不含生长素的固体培养基上诱导生根,形成完整植株,再将完整植株移入营养钵中或土壤中.这种生根方式代价较高,而且每株无根苗的转移均需在无菌条件下操作,不但花费很大的劳力,而且由于污染机会的增多将增加无菌苗染病死亡的机率.所以说,无根苗的诱导生根仍是茶树组织培养中的重要问题之一.为此,作者进行了无根苗水培诱导生根的研究,已先后从碧云、福鼎大白茶、阿萨姆种、龙井43等10余个品种的无根苗诱导生根. Since the 1970s, tea tree tissue culture as an important means of rapid propagation and genetic breeding has gained wide attention in the field of tea tree breeding. Many Chinese and foreign scientists use tea seed cotyledons, mature embryos, immature embryos, anthers, petioles and stem cortex Organs and tissues were used as explants and all the plants were obtained through cultivation.During the actual culture, the buds were often induced to differentiate first.Then the differentiated shoots were transferred to solid with low or no auxin Inducing rooting on medium, forming a complete plant, and then moving the whole plant into a nutrient bowl or soil This rooting method is costly and the transfer of rootless shoots per plant needs to be operated under sterile conditions, not only costly Of the labor force, but also because of increased opportunities for contamination will increase the chances of death of sterile seedlings disease, so the rooting of rootless seedlings is still one of the important problems in the tissue culture of tea tree.To this end, Rooting studies have rooted from rootless seedlings of more than 10 varieties of Pikun, Fuding Dabaicha, Assam, Longjing 43 and so on.