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目的：提供革螨传播肾综合征出血热病毒（ＨＦＲＳＶ）的分子生物学证据。方法：用上海真厉螨与柏氏禽刺螨叮咬ＨＦＲＳＶ接种乳小鼠，取叮咬后ｄ１０及ｄ１００以上的上海真厉螨与柏氏禽刺螨冰冻切片，用地高辛标记ＨＦＲＳＶｃＤＮＡ核酸探针原位分子杂交检测其体内病毒ＲＮＡ。结果：叮咬野鼠型（７６－１１８株）接种乳小鼠后ｄ１０上海真厉螨检出病毒ＲＮＡ，在细胞浆、细胞核中及核膜上呈细密颗粒状或全浆阳性，见于生殖器官细胞、胃及支囊上皮细胞及脑皮质细胞；叮咬家鼠型（ＵＲ株）病毒接种乳小鼠后ｄ１２螨亦检出病毒ＲＮＡ，病毒的细胞组织分布与野鼠型相同；分别检测野鼠型螨３１只和家鼠型２３只，其中阳性分别为１７只和１０只；叮咬野鼠型病毒接种乳小鼠后ｄ１３２，家鼠型ｄ１０２螨仍能检出病毒ＲＮＡ。柏氏禽刺螨叮咬野鼠型病毒接种乳小鼠ｄ１０，检测螨２０只，其中阳性１２只，病毒ＲＮＡ主要分布于生殖器官细胞内。结论：上海真厉螨与柏氏禽刺螨均可经叮咬获得ＨＦＲＳＶ，上海真厉螨可分别感染野鼠型和家鼠型ＨＦＲＳＶ，野鼠型在螨群体内至少可维持１３２ｄ，家鼠型１０２ｄ，具有贮存两型病毒的作用，是ＨＦＲＳＶ的适宜媒介和贮存宿主，在动物宿主间交叉传播两型ＨＦＲＳＶ中?
OBJECTIVE: To provide molecular biology evidences that leather mite transmission of hemorrhagic fever with renal syndrome virus (HFRSV). Methods: The mice were inoculated with HFRSV by bites of Trichoplusia nigerians and Platycodon pallidum. Frozen sections of Trichoplusia nippon and Apodemus patens above d10 and d100 after bites were labeled with digoxigenin, and labeled with HFRSV DNA probe in situ Hybridization to detect its viral RNA in vivo. Results: After being inoculated with the mice, the bovine wild type mouse (76-118 strain) detected the viral RNA at d10, and showed fine granular or whole plasma positive in the cytoplasm, nucleus and nuclear membrane, which were found in reproductive organs, Gastric and bronchial epithelial cells and cerebral cortical cells. The virus RNA was also detected in d12 mite after mice were inoculated with the mouse strain of house-bourgeon (UR strain) virus. The distribution of the virus cells was the same as that of the wild-type mice. Thirty-one And 23 domestic mice, of which 17 were positive and 10 mice; bite wild-mouse virus inoculated mice d132, domestic mice d102 mite still detectable viral RNA. Parkinson’s pteronyssinus biting wild-type virus inoculated mice d10, 20 mites were detected, of which 12 were positive, viral RNA mainly distributed in the reproductive organs cells. CONCLUSION: HFRSV can be obtained by biting bites of Trichoplusia nigerians and Platanus spp. Both of them can infect wild-type and domesticated HFRSV. The wild-type mites can maintain at least 132 days in mite population and 102 days in domestic mice, The role of storage of two types of viruses, HFRSV is the appropriate vector and storage host, cross-transmission of animal HFRSV in two types?