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目的 :研究“疗胃煎剂”对胃粘膜慢性损伤的防护作用。方法 :应用 2级 Wistar大鼠灌胃接种 SSl Hp菌株 ,灌胃 3%水杨酸钠溶液 ,交替自由饮用 5 %乙醇与 5 m mol/ L 去氧胆酸钠溶液及饥饱失常的综合方法制备 Hp相关 CAG动物模型。模型制备成功后均先行根除 Hp,进行“疗胃煎剂”治疗 ,并以三九胃泰作对照 ,12周疗程结束后 ,进行 Hp感染 ,组织病理学检查及血清 EGF及粘膜氨基己糖含量检测。结果 :“疗胃煎剂”中、高剂量组大鼠胃粘膜固有层、粘膜表面上皮层、PAS(+ )层厚度较模型自然恢复组明显增加 (P <0 .0 1) ,其中粘膜固有层与表面上皮层厚度增加明显优于三九胃泰 (分别 (P <0 .0 5 ) ,已接近正常组水平 (P >0 .0 5 )。“疗胃煎剂”与三九胃泰组均有增加 PAS(+ )层厚度的作用 (P <0 .0 5 )。“疗胃煎剂”中剂量组粘膜氨基己糖含量较模型自然恢复组明显增加 (P <0 .0 5 )。各治疗组血清 EGF浓度均较模型自然恢复组明显下降。结论 :“疗胃煎剂可以增加氨基己糖含量及粘膜表面上皮层厚度 ,具有增强粘膜屏障功能的作用。
Objective : To study the protective effect of “Stomach Decoction” on chronic gastric mucosal injury. METHODS: A comprehensive method was used to intragastrically inoculate SS1 Hp strains with grade 2 Wistar rats and to infuse 3% sodium salicylate solution, alternately drink 5% alcohol and 5 m mol/L sodium deoxycholate solution and hunger-saturation alternately. A Hp-related CAG animal model was prepared. After the successful preparation of the model, Hp was eradicated and “Stomach Decoction” was used for treatment. The Sanjiuweitai was used as a control. After 12 weeks of treatment, Hp infection was performed, histopathological examination and serum EGF and mucosal hexosamine levels were performed. Testing. RESULTS: The gastric mucosa lamina propria, mucosal surface epithelial layer, and PAS(+) layer thickness in the “Stomach Decoction” group were significantly higher than those in the natural recovery group (P < 0.01). The thickness of the layer and surface epithelium increased significantly better than Sanjiu Weitai (respectively (P < 0.05), and it was close to the normal group (P > 0.05). “Stomach decoction” and Sanjiu Weitai Both groups increased the thickness of PAS(+) layer (P < 0.05), and the mucosal hexose level in the middle-dose group of “Stomach Decoction” was significantly higher than that in the natural recovery group (P <0.05). The concentration of serum EGF in each treatment group was significantly lower than that in the model natural recovery group.Conclusion: "Stomach treatment decoction can increase the content of hexosamine and mucosal surface epithelial thickness, and it has the function of enhancing mucosal barrier function.