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This paper studies a conformal invariance and an integration of first-order differential equations. It obtains the corresponding infinitesimal generators of conformal invariance by using the symmetry of the differential equations, and expresses the differential equations by the equations of a Birkhoff system or a generalized Birkhoff system. If the infinitesimal generators are those of a Noether symmetry, the conserved quantity can be obtained by using the Noether theory of the Birkhoff system or the generalized Birkhoff system.