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目的探讨经皮外用异黄酮霜剂对紫外线照射所致皮肤损伤的作用及机制。方法雄性ICR小鼠随机分成4组(每组12只):对照组小鼠裸露皮肤仅接受纯基质涂布;紫外光照(UV)组、2%异黄酮组和3%异黄酮组小鼠裸露皮肤分别涂布纯基质、含2%或3%异黄酮的药物预处理15 min后,再先后接受长波紫外线(UVA,1.55J/cm2,18min)和中波紫外线(UVB,0.95J/cm2,11min)照射,制作紫外照射损伤的皮肤模型;连续7d后,取被照射部位皮肤组织行H-E染色,观察小鼠皮肤形态学和组织学变化情况,并测定小鼠皮肤组织中炎症因子白介素(IL)-1β、IL-6和肿瘤坏死因子α(TNF-α)的含量以及脂质过氧化产物丙二醛(MDA)、超氧化物歧化酶(SOD)和过氧化氢酶(CAT)的含量。结果 (1)UV组小鼠皮肤呈现红斑、结痂等改变,真皮层可见炎症细胞浸润,弹性纤维断裂;经2%或3%异黄酮预处理后,小鼠皮肤色泽均匀,镜下角质层无明显增厚,真皮层内胶原纤维排列较为整齐,无明显炎症细胞浸润。(2)与对照组小鼠相比,UV组小鼠皮肤组织中炎症因子IL-1β、IL-6和TNF-α的含量均增加(P<0.05);给予异黄酮预处理能够减弱紫外线照射所致小鼠皮肤组织中IL-1β、IL-6和TNF-α含量的改变(P<0.05,P<0.01)。(3)与对照组相比,UV组小鼠被照射皮肤组织中MDA含量增加(P<0.05),SOD和CAT含量均降低(P<0.05);给予对照异黄酮预处理能够减弱紫外线照射所致小鼠皮肤组织中MDA、SOD和CAT含量的改变(P<0.05,P<0.01)。结论异黄酮能够通过减轻小鼠皮肤组织脂质过氧化反应、氧化应激和炎症反应预防紫外线照射所致皮肤光老化损伤。
Objective To investigate the effect and mechanism of transdermal topical isoflavone cream on skin damage caused by ultraviolet radiation. Methods Male ICR mice were randomly divided into 4 groups (12 mice in each group): bare skin of mice in the control group were coated with pure stroma alone; mice exposed to ultraviolet light (UV), 2% isoflavone and 3% isoflavone After the skin was coated with pure matrix and pretreated with 2% or 3% isoflavone for 15 min, the skin was irradiated with UVA (UVA, 1.55J / cm2,18min) and UVB (UVB, 0.95J / cm2, 11min). The skin of the irradiated area was stained with hematoxylin-eosin (HE) to observe the morphological and histological changes of the skin of mice, and the levels of interleukin (IL) ) -1β, IL-6 and tumor necrosis factor-α (TNF-α) and the contents of malondialdehyde (MDA), superoxide dismutase (SOD) and catalase (CAT) . Results (1) The skin of mice in UV group showed erythema, crusting and other changes. The infiltration of inflammatory cells and elastic fibers were observed in the dermis. After pretreatment with 2% or 3% isoflavone, the mice had uniform skin color and stratum corneum No significant thickening, dermis collagen fibers arranged in a more orderly, no significant inflammatory cell infiltration. (2) Compared with the control group, the levels of IL-1β, IL-6 and TNF-αin the skin of UV-treated mice increased (P <0.05); the pretreatment with isoflavone decreased the UV-irradiation The changes of IL-1β, IL-6 and TNF-α in mouse skin tissue were significantly different (P <0.05, P <0.01). (3) Compared with the control group, the content of MDA and the contents of SOD and CAT in the skin of irradiated mice in UV group increased (P <0.05), while those in control group were decreased (P <0.05) The changes of MDA, SOD and CAT in mouse skin tissue (P <0.05, P <0.01). Conclusion Isoflavones can prevent skin photo-aging injury induced by ultraviolet radiation by reducing lipid peroxidation, oxidative stress and inflammatory reaction in skin of mice.