目的用大鼠坐骨神经压迫性损伤(Chronic Constriction Injury,CCI)痛觉模型,在实验性坐骨神经损伤后,在损伤局部即刻应用布比卡因,观察其对大鼠痛觉过敏行为及背根节(dorsal root ganglion,DRG)初级感觉神经元电泳速率的影响,并探讨其内在联系。方法术后5-20天,通过行为学实验方法观察CCI模型组与CCI+布比卡因药物组大鼠的两后肢热痛觉过敏行为的差异;随后观察两组大鼠的损伤神经元与对侧正常神经元的细胞电泳速率差异。结果CCI大鼠的损伤侧肢体的热痛敏回避反射潜伏期较对侧明显缩短,即表现为痛觉过敏;损伤神经元细胞电泳速率明显快于对侧正常神经元;而CCI+布比卡因组大鼠,损伤侧肢体的回避反射潜伏期与对侧无差异,损伤神经元的细胞电泳速率与对侧正常神经元无差异。结论神经损伤早期应用布比卡因,可阻止随后进一步发展为慢性痛,后期无热痛觉过敏行为异常,且损伤神经元的细胞电泳速率并不因神经损伤而发生改变。可见早期应用止痛剂,愈后良好。 OBJECTIVE To investigate the effects of chronic constriction injury (BPC) on the hyperalgesia and dorsal root (dorsal root) in rats with chronic constriction injury (CCI) pain model after experimental sciatic nerve injury. ganglion, DRG) primary sensory neurons electrophoresis rate, and to explore its intrinsic relationship. Methods From 5 to 20 days after operation, the difference of thermal hyperalgesia behavior between the two hindlimbs in CCI model group and CCI + bupivacaine group was observed by behavioral test. Then the injured neurons in the two groups were observed contralateral Differences in cell electrophoretic rates of normal neurons. Results The thermal pain-sensitive reflex reflex latency of injured limbs in CCI rats was significantly shorter than that in contralateral side, that is hyperalgesia. The rate of injured neurons was significantly higher than that of contralateral normal neurons. CCI + bupivacaine group There was no difference in avoidance reflex latency and contralateral contralateral latency between the injured and injured limbs, and the rate of cell electrophoresis in injured neurons and contralateral normal neurons. Conclusion Bupivacaine can be prevented in the early stage of nerve injury, which can prevent the subsequent development of chronic pain. There is no thermal hyperalgesia behavior in the late stage. The rate of cell electrophoresis in injured neurons does not change due to nerve injury. Visible early application of analgesics, the more good after.