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目的 :研究反义癌基因与抑癌基因联合抑制肝癌细胞生长的效果。方法 :利用基因重组方法构建携带 p1 6基因、p53基因的融合表达载体 (pc DNA1 6～ 53) ,利用基因合成仪体外合成 N- ras、C- myc反义寡核苷酸 ,并将 N- ras、C- myc反义寡核苷酸与 pc DNA1 6～ 53融合表达载体转染到人肝癌细胞系 SMMC772 1细胞中 ,观察肝癌细胞生长情况及软琼脂集落形成能力。结果 :联合治疗组肝癌细胞增殖速度最慢 ,软琼脂集落形成个数最少 ,只有 2个。结论 :利用相关基因联合治疗效果明显优于利用个别基因的治疗效果。
Objective: To study the combined effect of antisense oncogene and tumor suppressor gene on the growth of hepatoma cells. Methods: The recombinant plasmid p1 6 gene and p53 gene (pcDNA1 6 ~ 53) were constructed by gene recombination method. The antisense oligonucleotides N-ras and C-myc were synthesized by gene synthesizer in vitro and the N- ras, C-myc antisense oligonucleotides and pc DNA1 6 ~ 53 fusion expression vector were transfected into human hepatoma cell line SMMC772 1 cells to observe the growth of hepatoma cells and soft agar colony forming ability. Results: The proliferation rate of hepatocarcinoma cells in the combination therapy group was the slowest, with the smallest number of soft agar colonies, only two. Conclusion: The combination of related gene therapy is better than the use of individual gene therapy.