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目的检测胃癌组织中ＨＯＸＢ６基因是否存在异常甲基化，并探讨其与基因表达及临床因素的关系。方法用联合重硫酸盐限制性分析法（ＣＯＢＲＡ法）对７种胃癌细胞系和７３例胃癌患者的胃镜下活检组织进行ＨＯＸＢ６基因的甲基化定量检测，并采用逆转录聚合酶链反应方法检测基因的表达及脱甲基化处理后基因的再表达。结果７种胃癌细胞系中有４种异常甲基化，在异常甲基化的细胞系基因表达减少或消失，并且脱甲基化处理后基因可以重新表达。７３例胃癌患者的癌部位和非癌部位的活检组织中，２０例癌组织甲基化阳性（２７．４０％），而非癌组织中无１例阳性。结论胃癌中ＨＯＸＢ６基因的异常甲基化与基因表达抑制密切相关，并且脱甲基化后基因可以再表达；胃癌组织中ＨＯＸＢ６基因异常甲基化具有高度特异性，可作为肿瘤标志物用于临床诊断。
Objective To detect the abnormal methylation of HOXB6 gene in gastric cancer and to explore its relationship with gene expression and clinical factors. Methods HOXB6 gene methylation was quantitatively detected by gas chromatography in biopsy tissues from 7 gastric cancer cell lines and 73 gastric cancer patients by COBRA method and detected by reverse transcription polymerase chain reaction Gene expression and demethylation of genes after re-expression. Results Four of the seven gastric cancer cell lines were abnormally methylated, and the gene expression in the abnormally methylated cell lines decreased or disappeared, and the gene was re-expressed after demethylation. Among the 73 cases of gastric cancer and 20 cases of non-cancerous parts of the biopsy tissue, methylation was positive in 20 cases (27.40%), but none in non-cancerous tissues. Conclusions Abnormal methylation of HOXB6 gene in gastric cancer is closely related to the inhibition of gene expression, and the gene after demethylation can be re-expressed. Abnormal methylation of HOXB6 gene in gastric cancer is highly specific and can be used as a tumor marker in clinical diagnosis.