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目的:使用Luminex NxTAG? RPP试剂盒检测广州地区呼吸道感染样本，总结近一年广州地区呼吸道感染病原谱，为呼吸道感染临床诊疗和疾病防控工作提供参考依据。方法:采集2018年12月到2019年11月广东省妇幼保健院和广东省第二人民医院共484例呼吸道感染样本，采用QIAamp MinElute Virus Spin Kit(50)进行总核酸提取，采用Luminex NxTAG? RPP试剂盒检测样本中的病原体；采用Excel 2016对数据进行作图，采用SPSS19.0软件进行统计分析。结果:在484份标本中，病原体核酸阳性样本有397份，总阳性率为79.96%，感染以甲型流感病毒2009型(influenza A H1N1pdm09 virus, H1N1 2009)、鼻病毒(rhinovirus, RhV)、偏肺病毒(metapneumovirus, MPV)为主。在年龄分布中，儿童组的H1N1 2009、合胞病毒(respiratory syncytial virus, RSV)、副流感病毒(parainfluenza Virus, PIV)、MPV、RhV、博卡病毒(bocavirus，BoV)阳性率相对较高；成人组的乙型流感(influenza B virus，FLuB)阳性率相对较高。H1N1 2009、RSV、MPV、BoV在门诊患者中的阳性率较高；FLuB在住院患者阳性率相对较高。在不同症状的患者病原体检测中，咳嗽与咳痰症状患者的样本RSV阳性率较高；而鼻塞症状患者的样本冠状病毒(coronaviruses, CoV)、MPV、RhV阳性率较高。大多数病原体在冬春季检出率偏高。结论:H1N1 2009、RhV、MPV为2019年广州呼吸道感染的重要病原体，加强对这些病原体的研究与监测，可作为广州呼吸道感染性疾病的防控重点。“,”Objective:Luminex NxTAG? RPP kit was used to detect respiratory tract infection samples in Guangzhou, and the pathogen spectrum of respiratory tract infection in Guangzhou in the past year was summarized to provide reference for clinical diagnosis, treatment and disease prevention and control of respiratory tract infection.Methods:A total of 484 respiratory infection samples were collected from December 2018 to November 2019 from Guangdong Maternal and Child Health Hospital and The Second People's Hospital of Guangdong Province. QIAamp MinElute Virus Spin Kit(50) was used to extract total nucleic acid, and Luminex NxTAG? RPP Kit was used to detect pathogens in the samples. Excel 2016 was used to draw the data and SPSS19.0 software was used for statistical analysis.Results:Among the 484 samples, 397 were found to be positive, with A total positive rate of 79.96%. Infection included Influenza A H1N1pdm09 virus (H1N1 2009), rhinoviruses (RhV), Metapneumovirus (MPV). In the age distribution, the positive rates of H1N1 2009, Respiratory syncytial virus (RSV), Parainfluenza virus (PIV), MPV, RhV and Bocavirus (BoV) were relatively high in the children group. The positive rate of Influenza B virus (FLuB) in adult group was relatively high. The positive rates of H1N1 2009, RSV, MPV and BoV were higher in outpatients. The positive rate of FLuB was relatively high in hospitalized patients. In the pathogen detection of patients with different symptoms, the RSV positive rate of samples from patients with cough and sputum symptoms was higher. The positive rates of Coronaviruses (CoV), MPV and RhV samples from patients with nasal obstruction were higher. The detection rate of most pathogens is high in winter and spring.Conclusions:H1N1 2009, RhV and MPV are the important pathogens of respiratory tract infections in Guangzhou in 2019. Strengthening the research and monitoring of these pathogens can be the focus of the prevention and control of respiratory tract infectious diseases in Guangzhou.