目的:建立磷钼钨酸/干酪素分光光度法测定不同产地藏药余甘子中总鞣质含量的方法。方法:以没食子酸为对照品,磷钼钨酸为显色剂,检测波长760 nm,测定8个不同产地余甘子中总鞣质含量。结果:没食子酸在0.306～2.448 mg.L-1与吸光度呈良好线性关系(r=0.999 1),平均加样回收率99.27%,RSD 2.30%。不同产地余甘子药材总鞣质含量差别较大(6.72%～11.95%),印度和尼泊尔产余甘子中总鞣质含量最高。结论:该方法简单、准确、重复性好,可用于测定余甘子中总鞣质含量。 Objective: To establish a phosphomolybdotungstic acid / casein spectrophotometric method for the determination of total tannin content in the Tibetan medicine Phyllanthus emblica from different areas. Methods: Using gallic acid as reference substance, phosphomolybdotungstic acid as chromogenic reagent, the detection wavelength was 760 nm, and the content of total tannin in amla of eight different producing areas was determined. Results: There was a good linear relationship between the concentration of gallic acid and absorbance at 0.306 ~ 2.448 mg · L-1 (r = 0.999 1). The average recovery was 99.27% ​​and the RSD was 2.30%. The content of total tannin in the different producing areas varies greatly (6.72% ~ 11.95%), the content of total tannin in the amla of India and Nepal is the highest. Conclusion: The method is simple, accurate and reproducible. It can be used to determine total tannin in Phyllanthus emblica.