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nm23-H1基因与肺癌的侵袭与转移密切相关,但是其作用的分子机制尚不清楚,为研究nm23-H1基因的功能,筛选并鉴定了nm23-H1基因缺失人肺癌细胞株及其生物学特性.应用Southernblot,RT-PCR和West-ernblot检测9株人肺癌细胞株中nm23-H1基因的存在状态及其生物学行为.结果发现发现人大肺癌细胞株L9981中存在nm23-H1等位基因的杂合性缺失,与其同源的NL9980及其它7株肺癌细胞株中nm23-H1基因均以杂合子的形式存在;并且L9981细胞株的增殖能力、克隆形成能力、体外侵袭力,裸鼠体内成瘤性及移植瘤肺转移的能力均显著高于NL9980.研究结果显示nm23-H1基因的缺失可能与L9981细胞株恶性表型和高转移潜能密切相关.
The molecular mechanism of nm23-H1 gene is closely related to the invasion and metastasis of lung cancer. However, the molecular mechanism of its role is unclear. To study the function of nm23-H1 gene, the human lung cancer cell line with nm23-H1 gene deletion and its biological characteristics were screened and identified Southern blot, RT-PCR and West-ernblot were used to detect the existence and biological behavior of nm23-H1 gene in 9 human lung cancer cell lines.It was found that human nm23-H1 allele was present in L9981 human lung cancer cell line The co-localization of NL9980 and other seven lung cancer cell lines nm23-H1 gene exist in the form of heterozygotes; and L9981 cell proliferation, clonality, in vitro invasiveness, tumor formation in nude mice And lung metastasis of xenografts were significantly higher than NL9980.The results show that the loss of nm23-H1 gene may be closely related to the malignant phenotype and high metastatic potential of L9981 cell line.