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目的建立人血浆中利奈唑胺浓度测定的高效液相色谱法。方法以苯巴比妥为内标,血浆样品用乙腈沉淀蛋白,采用LunaC18柱(250mm×4.6mm,5μm)分析。流动相为乙腈-水(25:75),流速为1.0mL·min-1,检测波长251nm,柱温35℃。结果本法在0.1～40mg·L-1内线性关系良好(r=0.9999),检测限为0.05mg·L-1,日内、日间RSD均小于4%。结论本方法简单、灵敏、准确,适用于利奈唑胺临床血药浓度监测及人体药动学研究。
Objective To establish a method for the determination of linezolid in human plasma by high performance liquid chromatography. Methods Phenobarbital was used as an internal standard and plasma samples were precipitated with acetonitrile and analyzed on a Luna C18 column (250 mm × 4.6 mm, 5 μm). The mobile phase consisted of acetonitrile-water (25:75), the flow rate was 1.0 mL · min-1, the detection wavelength was 251 nm and the column temperature was 35 ℃. Results The linearity was good (r = 0.9999) in 0.1 ~ 40 mg · L-1 and the detection limit was 0.05 mg · L-1. The daily RSD and intraday RSD were less than 4%. Conclusion The method is simple, sensitive and accurate and is suitable for the clinical monitoring of linezolid and its pharmacokinetics.