论文部分内容阅读
木文研究了多种氨基酸、乙醇胺和甲基乙醇胺对细胞摄取胆碱和合成磷脂酰胆碱(PC)的影响,发现多种氨基酸非竞争性地抑制细胞摄取胆碱。含胆碱代谢物的分析显示胆碱转变成CDP-胆碱,随之形成PC均不受氨基酸影响。乙醇胺竞争性地抑制胆碱摄取,且存在剂量依赖关系。乙醇胺能明显抑制胆碱激酶活性,但细胞内胆碱和磷酸胆碱的代谢池并不改变,提示乙醇胺不影响胆碱转变成磷酸胆碱。根据CDP-胆碱和PC的比放射性分布,乙醇胺也不影响PC的生物合成。甲基乙醇胺抑制胆碱摄入的程度强于乙醇胺,并抑制胆碱激酶和CTP:磷酸胆碱胞苷转移酶活性,含胆碱代谢物以CDP-胆碱下降最显著;提示甲基乙醇胺不仅抑制胆碱摄入而且还干扰了CDP-胆碱通路。
Mu Wen studied a variety of amino acids, ethanolamine and methyl ethanolamine uptake of choline cells and the synthesis of phosphatidylcholine (PC), found that a variety of amino acids non-competitive inhibition of cell uptake of choline. Analysis of choline-containing metabolites showed that conversion of choline to CDP-choline followed by PC formation was not affected by amino acids. Ethanolamine competitively inhibited choline uptake in a dose-dependent manner. Ethanolamine significantly inhibited choline kinase activity, but the metabolic pool of intracellular choline and phosphocholine did not change, suggesting that ethanolamine did not affect the conversion of choline to phosphorylcholine. Ethanolamine also does not affect PC biosynthesis based on the specific radioactivity of CDP-choline and PC. Methylethanolamine inhibited choline uptake to a greater extent than ethanolamine and inhibited both choline kinase and CTP: phosphorylcholine cytidine transferase activity, with choline metabolites most significantly decreasing with CDP-choline; suggesting that methyl ethanolamine not only Inhibition of choline uptake also interferes with the CDP-choline pathway.