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目的:研究麻黄桂枝配伍对小鼠行为活动和中枢多巴胺神经递质水平的影响。方法:40只KM小鼠按体重随机分为空白对照组、麻黄组(10 g/kg)、麻黄-桂枝3:2组(10 g/kg+6.67 g/kg)、麻黄-桂枝3:1组(10 g/kg+3.33 g/kg)、桂枝组(6.67 g/kg),灌胃给药7 d,进行旷场实验;灌胃给药12 d,进行避暗实验,24 h后重复试验;灌胃给药14 d,免疫组化法检测小鼠大脑海马、纹状体、皮层多巴胺(DA)、多巴胺转运体(DAT)含量,HE染色观察小鼠大脑海马、纹状体、皮层病理变化。结果:与空白对照组比较,麻黄组(10 g/kg)小鼠自主活动增加,静止时间降低,运动时间及路程增加,小鼠进入暗箱的潜伏期缩短,错误次数和错误只数增加,大脑海马、纹状体、皮层中DA、DAT含量显著降低,病理检测有明显损伤;桂枝组(6.67 g/kg)与空白对照组相比无明显差异。与麻黄组(10 g/kg)相比,麻黄-桂枝3:2组(10 g/kg+6.67 g/kg)、麻黄-桂枝3:1组(10 g/kg+3.33 g/kg)小鼠自主活动减少,静止时间增加,运动时间及路程降低,小鼠进入暗箱的潜伏期延长,错误次数和错误只数减少,小鼠大脑海马、纹状体、皮层中DA、DAT含量增加,其病理损伤均有不同程度改善。结论:桂枝能够抑制麻黄所致的中枢的兴奋作用,改善小鼠行为活动的不良影响,减少中枢多巴胺能神经元损伤,且具有一定的量效关系,随着桂枝比例的增加,桂枝拮抗麻黄中枢毒副作用更为显著,其作用机制可能与增加多巴胺神经递质含量有关。
Objective: To investigate the effects of compatibility of Ephedra camai against behavioral activity and central dopamine neurotransmitter in mice. Methods: 40 KM mice were randomly divided into blank control group, ephedrine group (10 g / kg), ephedra - Guizhi 3: 2 group (10 g / : Group 1 (10 g / kg + 3.33 g / kg) and Guizhi group (6.67 g / kg) for 7 days. h after repeated intragastric administration; 14 days after intragastric administration, the content of dopamine (DA) and dopamine transporter (DAT) in the hippocampus, striatum and cortex were detected by immunohistochemistry. The hippocampus, Body, cortical pathological changes. Results: Compared with the blank control group, the mice in the ephedra group (10 g / kg) increased autonomic activity, decreased the rest time, increased the exercise time and distance, shortened the incubation period for the mice into the dark box, increased the number of errors and errors, , The content of DA and DAT in the striatum and cortex decreased significantly and the pathological examination showed obvious damage. There was no significant difference between the Guizhi group (6.67 g / kg) and the blank control group. Compared with ephedra group (10 g / kg), Ephedra sinicola 3: 2 group (10 g / kg + 6.67 g / kg) ) Decreased autonomic activity, increased resting time, reduced exercise time and distance, increased the latency of mice entering the dark box, reduced the number of errors and errors, and increased the contents of DA and DAT in the hippocampus, striatum and cortex of mice, The pathological changes have improved to varying degrees. Conclusion: Guizhi can inhibit the central excitement induced by ephedra, improve the adverse effects of behavioral activities and reduce the damage of central dopaminergic neurons, and have a dose-effect relationship. With the increase of the proportion of Guizhi, Guizhi Antagonism of ephedra central toxicity side effects are more significant, its mechanism may be related to increased dopamine neurotransmitter content.