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目的探讨过氧化物酶体增殖物激活受体γ(PPARγ)的配体15-脱氧-△~(12,14)-前列腺素J2(15d-PGJ_2)对人胃癌MGC803细胞生长的影响。方法逆转录-聚合酶链反应(RT-PCR)检测过氧化物酶体增殖物激活受体γ(PPARγ)及Survivin mRNA表达,Western blot检测PPARγ、Sur- vivin蛋白的表达。噻唑蓝(MTT)比色法及流式细胞术检测增殖、凋亡。Hoechst33342染色观察凋亡的形态学变化。结果PPARγmRNA和蛋白在MGC803细胞均表达,15d-PGJ_2(5、10、20、30和40μmol/L)抑制MGC803细胞增殖和诱导其凋亡,增殖抑制率和凋亡率随浓度的增加和时间的延长而升高。MGC803细胞Survivin mRNA和蛋白表达随15d-PGJ_2作用浓度的升高而降低。结论15d-PGJ_2可能通过诱导凋亡抑制人胃癌MGC803细胞的生长,Survivin mRNA和蛋白表达变化在此过程中起重要作用,提示15d-PGJ_2可能对治疗胃癌有效。
Objective To investigate the effect of 15-deoxy-Δ12,14-prostaglandin J2 (15d-PGJ_2), a ligand of peroxisome proliferator-activated receptor γ (PPARγ), on the growth of human gastric cancer MGC803 cells. Methods The expression of peroxisome proliferator - activated receptor γ (PPARγ) and Survivin mRNA were detected by reverse transcription - polymerase chain reaction (RT - PCR). The protein expressions of PPARγ and Survivin were detected by Western blot. Thiazolyl blue (MTT) colorimetry and flow cytometry were used to detect proliferation and apoptosis. Hoechst33342 staining was used to observe the morphological changes of apoptosis. Results PPARγmRNA and protein were all expressed in MGC803 cells. 15d-PGJ_2 (5, 10, 20, 30 and 40μmol / L) inhibited the proliferation and induced apoptosis of MGC803 cells with increasing concentration and time Extend and rise. Survivin mRNA and protein expression in MGC803 cells decreased with the increase of 15d-PGJ_2 concentration. Conclusion 15d-PGJ_2 may inhibit the growth of human gastric cancer cell line MGC803 by inducing apoptosis. The changes of Survivin mRNA and protein expression play an important role in this process, suggesting that 15d-PGJ_2 may be effective in treating gastric cancer.