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1985—1986年的实验结果表明,以初发和复发的间日疟病例为传染源,将病原体蚊传给子代,其潜伏期在蚊传过程中时长时短[见本刊7(1):28～31,1989],未能分离出潜伏期稳定长或稳定短的虫株,从而得出我国温带地区间日疟潜伏期的长短取决于进入宿主子孢子量的结论。但是以阳性蚊涎腺内子孢子的感染度和叮咬志愿者的阳性蚊数判断进入人体的子孢子量,不一定十分精确可靠。所以,在实验室内反复摸索了多种分离子孢子的方法,终
The experimental results from 1985 to 1986 showed that mosquitoes of pathogens were transmitted to offspring from newly infected and recurrent malaria cases and their incubation period was long and short during mosquito transmission (see 7 (1): 28 ~ 31, 1989], failed to isolate a long or short incubation period stable strains of insects, and draw the conclusion that the length of incubation period in temperate regions of China depends on the amount of host sporozoites. However, it is not necessarily accurate and reliable to judge the amount of sporozoites entering the human body by the degree of infection of the sporozoites in the positive mosquito salivary glands and the positive mosquito numbers of the bite volunteers. Therefore, in the laboratory repeatedly explored a variety of methods of separation of sporozoites, and finally