流行病学研究发现空气中羊毛尘和谷物尘与作业工人肺部炎症和支气管上皮细胞损伤有明显关系。为了进一步探讨这一问题,本研究采用体外细胞培养方法观察此2种粉尘对肺上皮细胞和肺泡巨噬细胞的直接毒性作用。羊毛尘系指在羊毛加工过程中用采样器收集到的空气中漂浮的粉尘。表面羊毛尘来自羊毛加工厂周围墙壁和地面上粘附的羊毛尘表层部分。谷物尘采自谷仓壁内表面粘附的粉尘,经筛选,采用粒径在45μm以下的尘粒。为了探讨损伤性因素是否是可溶性,分别制备了羊毛 Epidemiological studies have found that wool dust and cereal dust in the air are significantly related to lung inflammation and bronchial epithelial cell injury in workers. In order to further explore this issue, this study uses in vitro cell culture methods to observe the two kinds of dust on lung epithelial cells and alveolar macrophages direct toxicity. Wool dust refers to dust floating in the air collected by a sampler during wool processing. Surface wool dust comes from the surface of the woolen dust that adheres to the walls around the wool processing plant and to the ground. Grain dust from the inner surface of the barn wall adhesion of dust, after screening, the use of particle size below 45μm dust particles. In order to investigate whether the damage factors are soluble, wool was separately prepared