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对已建立的人-人-鼠杂交瘤2D_5细胞株分泌的人单克隆抗体(H-McAb)进行了免疫学特性分析。结果显示,H-McAb2D_5的相对亲和力为0.2mg/L;中和效价为1:160;Western-Blotting显示此McAb是针对55KD结构蛋白的;特异性阻断试验证明具有较好的特异性。
The immunological characteristics of human monoclonal antibody (H-McAb) secreted by established human-human-mouse hybridoma 2D_5 cell line were analyzed. The results showed that the relative affinity of H-McAb2D5 was 0.2 mg / L; the neutralization titer was 1: 160; Western-blotting showed that the McAb was against 55 kD structural protein; and the specific blocking test proved to be of good specificity.