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以3年生“早钟6号”枇杷(Eriobotrya japonica Lindl.)容器苗为试材,采用100.0 mmol/L钙调素拮抗剂三氟拉嗪(Trifluoperazine,TFP)的Hoagland营养液砂培法预处理24 h后,于-3℃人工气候室进行低温胁迫,研究钙调素拮抗剂TFP对低温胁迫下枇杷幼果AsA-GSH循环的影响。结果表明:TFP处理导致低温胁迫下枇杷幼果H2O2含量高于单一低温胁迫的LTS处理(Low Temperature Stress,LTS),而AsA和GSH含量低于LTS处理,且两者差异达显著水平(p<0.05);同时GR、DHAR、MDAR、APX活性也受到不同程度的抑制,其中对APX和GR活性的抑制作用较为明显。TFP预处理削弱了枇杷幼果低温胁迫后的抗氧化能力,加重了低温胁迫对幼果的伤害,表明低温胁迫下枇杷幼果的AsA-GSH循环受Ca2+.CaM信使系统的诱导和调控。
The seedlings of 3-year-old Eriobotrya japonica Lindl were used as test materials, and Hoagland nutrient solution sand culture with 100.0 mmol / L calmodulin antagonist Trifluoperazine (TFP) After pretreatment for 24 h, the cells were subjected to low temperature stress in a -3 ° C artificial climate chamber to study the effects of calmodulin antagonist TFP on AsA-GSH cycling in young loquat fruits under low temperature stress. The results showed that under low temperature stress, TFP treatment resulted in higher H2O2 content in young loquat fruits than in low temperature stress (LTS) treatments, while AsA and GSH contents were lower than LTS treatment (p < 0.05). At the same time, the activities of GR, DHAR, MDAR and APX were also inhibited to some extent, and the inhibitory effects on APX and GR activity were obvious. Pretreatment with TFP attenuated the antioxidative ability of young loquat fruits after low temperature stress and aggravated the injury of young fruits under low temperature stress. The results showed that AsA-GSH cycle in young loquat fruits was induced and regulated by Ca2 + .CaM messenger system under low temperature stress.