论文部分内容阅读
目的探讨柑橘素(Nari)对大鼠缺血再灌注心脏损伤作用后保护作用。方法将本研究中心56只雄性成年SD大鼠随机分为4组:对照组、Nari组、Nari+格列苯脲(Gli)组、Nari+5-羟基葵酸(5-HD)组;各组大鼠心脏给予30 min全心缺血、60 min再灌注;记录不同Nari浓度水平对再灌注心脏损伤作用,并评估其对氧自由基、热休克蛋白70水平的影响。结果心脏缺血/再灌注后60 min,2.5μmol/L以上Nari组大鼠心脏功能恢复优于对照组;Nari+Gli、Nari+5-HD大鼠心肌梗死面积、冠脉流出液LDH含量均高于10μmol/L Nari组大鼠;心脏缺血/再灌注后60 min,2.5μmol/L、5.0μmol/L、10.0μmol/L、20.0μmol/L Nari组心肌组织HSP70表达水平、SOD水平高于对照组,呈逐渐增加的趋势,心肌组织MDA水平低于对照组,呈逐渐降低的趋势,差异均有统计学意义(P<0.05)。结论柑橘素对心肌缺血再灌注心脏损伤具有保护作用。
Objective To investigate the protective effect of Nari on rat heart with ischemic reperfusion injury. Methods Fifty-six adult male Sprague-Dawley rats were randomly divided into 4 groups: control group, Nari group, Nari + Gli group and Nari + 5-HD group; The hearts of rats were given ischemia for 30 min and then reperfused for 60 min. The effects of different concentrations of Nari on heart injury were recorded and the effects on oxygen free radicals and heat shock protein 70 were evaluated. Results At 60 min after ischemia / reperfusion, cardiac function recovery was better in the Nari group than in the 2.5 μmol / L group. The myocardial infarct size and coronary effluent LDH content in Nari + Gli and Nari + 5-HD rats The expression of HSP70 in myocardium of 2.5μmol / L, 5.0μmol / L, 10.0μmol / L and 20.0μmol / L Nari groups was significantly higher than that in Nari group (10μmol / L Nari group) In the control group, there was a trend of increasing gradually. The level of MDA in myocardial tissue was lower than that in the control group, showing a trend of decreasing gradually. The difference was statistically significant (P <0.05). Conclusion Citrus has a protective effect on myocardial ischemia-reperfusion injury.