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目的:研究旋覆代赭汤对混合反流性食管炎(RE)家兔食管黏膜血管活性肠肽(VIP)、P物质(SP)和一氧化氮合酶(NOS2)水平的影响。方法:制备家兔RE模型,并进行常规HE染色观察食管黏膜病理改变,免疫组织化学技术检测兔食管黏膜VIP、SP、NOS2的水平。结果:与正常组比较,模型组家兔食管黏膜炎细胞浸润明显,VIP、NOS2表达明显升高(P<0.01),SP表达明显减弱(P<0.01);经中药治疗后全方组、甘升组、去苦降组、去升降相因组光镜下食管黏膜组织有不同程度的修复,其中全方组、甘升组食管黏膜中VIP的表达明显减弱,与模型组相比有显著性差异(P<0.01);与模型组比较,全方组、去升降相因组SP的表达显著增强(P<0.01);与模型组比较,全方组、去苦降组NOS2的表达显著减弱(P<0.01)。结论:旋覆代赭汤全方通过减少食管黏膜VIP和NOS2表达、增强SP表达调节胃肠运动,抑制胃肠内容物的反流和促进黏膜的修复,对RE治疗作用最好;而苦降组、升降相因组、去甘升组对RE治疗效果不佳。
Objective: To study the effect of Xuanfu Daizhitang on esophageal mucosal vasoactive intestinal peptide (VIP), substance P (SP) and nitric oxide synthase (NOS2) in rabbits with mixed reflux esophagitis (RE). Methods: Rabbit RE models were prepared. The pathological changes of esophageal mucosa were observed by HE staining. The levels of VIP, SP and NOS2 in rabbit esophageal mucosa were detected by immunohistochemistry. Results: Compared with the normal group, the infiltration of esophageal mucosal cells in rabbits in the model group was significant, the expressions of VIP and NOS2 were significantly increased (P <0.01), and the expression of SP was significantly decreased (P <0.01). After treatment with traditional Chinese medicine, In the ascending group and the descending group, the esophageal mucosa tissue was repaired to varying degrees by the phase-contrast phase group. The expression of VIP in the esophageal mucosa of the whole group and the ascending group was significantly weaker than that in the model group (P <0.01). Compared with the model group, the expression of SP in all the three groups increased significantly (P <0.01). Compared with the model group, the expression of NOS2 was significantly decreased (P <0.01). CONCLUSION: Xuanfu Daizhe decoction has the best effect on RE by reducing the expressions of VIP and NOS2 in esophageal mucosa, enhancing the expression of SP, regulating the gastrointestinal motility, inhibiting the reflux of gastrointestinal contents and promoting mucosal repair. However, Phase of the group, to the Gan Sheng group of RE treatment is not good.