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臭氧(O_3)是具有强氧化性的刺激性气体,是现代工业、交通运输业发达城市的主要污染物之一,也是光化学烟雾污染物的主要成分(约占90％)。为进一步探讨臭氧对生物体的遗传毒理效应,我们应用紫露草微核技术对此作了研究。 用南京植物研究所引进的美国沼泽紫露草3号敏感品种。检测原理是根据紫露草的花粉母细胞在减数分裂过程中,染色体受到致突变物作用后发生断裂,在四分体期形成微核,观察四分体中的微核数,用微核率来评价环境中污染物的致突变性。
Ozone (O 3) is an irritant gas with strong oxidizing properties. It is one of the major pollutants in the modern industrialized and transportation developed cities and the main component (about 90%) of photochemical smog pollutants. In order to further explore the genotoxic effects of ozone on organisms, we applied this technique to the study. With the introduction of the Nanjing Institute of Botany American red swamp grass No. 3 sensitive varieties. Detection principle is based on the pollen mother cells of Mealya meiosis process, the chromosome by the mutagenic effect after the break, the formation of micronucleus in the quartile, observed in the quadrilateral micronucleus, the micronucleus rate to To evaluate the mutagenicity of pollutants in the environment.