研究秦巴山区野生牛肝菌多糖提取最佳工艺及含量测定最佳方法。采用L16(44)正交试验优化水提取牛肝菌多糖的工艺条件;采用单因素设计研究苯酚-硫酸法测定多糖的最佳条件。结果显示:提取牛肝菌多糖和含量测定的最佳工艺为:温度95℃,水提时间4 h,料液比1∶10;测定多糖的最佳条件为15%苯酚0.6 mL,浓硫酸4.0 mL。采用本试验方法可以充分提取牛肝菌多糖,秦巴山区野生牛肝菌多糖含量达到4.5%。 To study the best method and content determination of polysaccharide from Boletus edulis in Qinba Mountain area. The optimum conditions of extracting polysaccharides from Boletus edulis by L16 (44) orthogonal test were optimized. The optimal conditions for determination of polysaccharides by phenol-sulfuric acid method were studied by single factor design. The results showed that the optimum conditions for the extraction of polysaccharides were as follows: the temperature was 95 ℃, the time of water extraction was 4 h and the ratio of solid to liquid was 1:10. The optimal conditions for polysaccharides determination were as follows: 15% phenol 0.6 mL, concentrated sulfuric acid 4.0 mL. The method can be used to extract the polysaccharides of Boletus edulis, and the content of polysaccharides of Boletus edulis in Qinling-Bashan reaches 4.5%.