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目的:探索利多卡因对缺氧脑细胞保护作用的途径。方法:选用出生24小时以内的Wistar大鼠乳鼠,分离其大脑皮层细胞进行培养,用MTT方法观察细胞在缺氧条件下培养24小时及48小时后OD值的变化,然后将细胞分为正常培养组,缺氧培养组,缺氧培养十利多卡因组,分别测定培养48小时后培养液中乳酸及丙二醛的含量。结果:大鼠大脑皮层细胞在缺氧培养24小时后,其存活率同正常培养组相比无显著差别;缺氧培养48小时后,其存活率显著下降。缺氧培养48小时后,大鼠脑细胞培养液中丙二醛和乳酸水平显著升高,而利多卡因组的升高则不明显。结论:利多卡因有抑制由缺氧引起的大脑皮层细胞丙二醛和乳酸水平升高的作用。
Objective: To explore the protective effect of lidocaine on hypoxic brain cells. Methods: Wistar rat neonatal rats within 24 hours of birth were selected for culturing. The cells were cultured under hypoxia for 24 hours and 48 hours with MTT method. The cells were divided into normal Culture group, hypoxia culture group and hypoxia culture group, respectively. The contents of lactic acid and malondialdehyde in the culture medium were measured after 48 hours culture respectively. Results: The survival rate of rat cerebral cortex cells after hypoxia for 24 hours was not significantly different from that of normal group. The survival rate of rat cerebral cortex cells decreased significantly after 48 hours hypoxia. After hypoxia for 48 hours, the levels of malondialdehyde and lactate in rat brain cell culture medium increased significantly, while the increase in lidocaine group was not obvious. Conclusion: Lidocaine can inhibit the increase of malondialdehyde and lactate levels in cerebral cortex cells induced by hypoxia.