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目的检测健康人携带的沙门菌中沙门菌基因组岛(SGI1)的分布情况及特性,分析其与细菌耐药性的关系。方法采用PCR方法对5株第一类整合子阳性的沙门菌检测SGI1,分别检测其左侧、右侧部分和其中间的部分基因,以及环状形式和切除状态,通过接合转移及自然转化检测其可移动性。结果一株鼠伤寒沙门菌携带SGI1,位于thdF和int2之间,且其多重耐药区含aadA2,floR,tetG,blaPSE-1基因,存在环状形式及切除状态,且可通过自然转化方式转移至其他菌株。结论 SGI1可通过自然转化方式转移到其他菌株,介导多重耐药的传播。
Objective To detect the distribution and characteristics of Salmonella genomic islands (SGI1) carried by healthy human and to analyze the relationship between SGI1 and bacterial resistance. Methods Five strains of Salmonella integron positive in the first group were detected by PCR. The left and right part and the middle part of SGI1 were detected by PCR, and the ring form and excision status were detected. The positive rate of SGI1 was detected by junctional transfer and natural transformation Its mobility. Results A Salmonella typhimurium harboring SGI1 located between thdF and int2, and its multidrug resistance region contains aadA2, floR, tetG and blaPSE-1 genes, which exist in cyclic and excised status and can be transferred by natural transformation To other strains. Conclusion SGI1 can be transferred to other strains by natural transformation and mediated the spread of multi-drug resistance.