论文部分内容阅读
Objective: To study the influence of neoadjuvant chemotherapy on the expression of cyclin D1,Bcl-2,PCNA and Pgp in osteosarcoma cells and the relationship between the expression and tumor cell necrosis rate (TCNR) after chemotherapy.Methods: By using immunohistochemistry,the expression of cyclin D1,Bcl-2,PCNA and P-gp was detected in 23 cases of osteosarcoma and TCNR were calculated.Results: The pre-chemotherapy positive expression rate of cyclin D1,Bcl-2,PCNA and P-gp was 73.9%,69.6%,91.3% and 21.7%,respectively,and that post-chemotherapy positive expression rate was 52.1%,34.8%,43.5% and 56.5%,respectively.The positive expression rate of Bcl-2 and PCNA after chemotherapy was much lower than that before chemotherapy (P=0.039,0.034).After chemotherapy,the expression rate of P-gp was higher (P=0.021) and the expression of cyclin D1 had no statistically significant difference (P=0.180) comparing with that before chemotherapy.No correlation existed between the expression of cyclin D1,Bcl-2,PCNA,P-gp and TCNR before chemotherapy (P=0.155,0.371,1.000and 0.640).There was a negative correlation between the expression of Bcl-2,PCNA,P-gp and TCNR (P=0.00g,0.012 and0.015),but no relationship existed between the cyclin D1 and TCNR (P=0.100) after chemotherapy.Conclusion: Chemotherapy could inhibit proliferation and induce apoptosis of osteosarcoma cells.At the same time,due to the overexpression of the P-gp,the drug resistance of the osteosarcoma cells was increased.The detection of cyclin D1,Bcl-2,PCNA and P-gp in osteosarcoma samples before chemotherapy might not be used to predict the curative effect of the chemotherapy.