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以郑芝4号黑芝麻种子为材料,研究了人工加速老化处理对种子发芽指标、丙二醛(MDA)含量、过氧化物酶(POD)活性和超氧化物歧化酶(SOD)活性的影响。试验结果表明:随着种子老化加深,发芽势、发芽率、发芽指数、活力指数逐渐降低,丙二醛含量逐渐升高。另外,老化种子内POD活性和SOD活性变化的总趋势是随老化时间延长而降低。由此认为芝麻种子人工老化及劣变的主要机制与抗氧化酶活性降低和膜脂过氧化作用加剧有关。人工老化种子生活力出现快速下降的发芽势拐点是80%,其下降的趋势又与POD活性下降非常一致,POD可作为一个检测指标显示种子活力高低及丧失的特征。
The effects of artificial accelerated aging treatment on seed germination index, malondialdehyde (MDA) content, peroxidase (POD) activity and superoxide dismutase (SOD) activity of Zhengzhi No.4 black sesame seeds were studied. . The results showed that with the deepening of seed aging, the germination potential, germination rate, germination index, vitality index decreased gradually and MDA content increased gradually. In addition, the general trend for changes in POD activity and SOD activity in aged seeds is to decrease with increasing aging time. Therefore, the main mechanism of artificial aging and deterioration of sesame seeds is related to the decrease of antioxidant enzyme activity and the increase of membrane lipid peroxidation. The germination potential of the artificial aged seed with rapid decline appeared to be 80%. The declining tendency was in good agreement with the decline of POD activity. POD could be used as a test indicator to show the seed vigor and loss characteristics.